m perez losada

Marcos Perez-Losada

Title:
Assistant Professor, Computational Biology Institute
Faculty: Full-Time
Office:
305D
Address: Innovation Hall
Phone: 571-553-0243
Email:
mlosada@gwu.edu

Areas of Expertise

Phylogenetics, Infectious disease, Invertebrate Systematics, Molecular Evolution, HIV, Population Genetics, Bacterial Genomics, Conservation Genetics

Faculty member since 2013.

Current Research

Overview

My research focuses mainly on evolutionary questions related to invertebrate biodiversity, dynamics of human infectious diseases and host-microbe interactions. I am also interested in comparing methods of phylogenetic and population genetic analysis and data types to study evolutionary processes at both the species and population levels. My interest in invertebrate biodiversity centers around mollusks, crustaceans and earthworms, and encompasses the study of their taxonomic and morphological diversity, systematics, phylogeography and conservation. My research on human infectious diseases focuses on gonorrhea and HIV and explores the evolutionary and epidemiological factors driving the dynamics of human infectious diseases (phylodynamics). Recently, I have become interested in studying the evolutionary and ecological interactions between earthworms and their associated microbial fauna and the impact worms have on the structure and function of soil microbes.

Invertebrate Biodiversity

We have generated the first comprehensive phylogenies of charismatic groups such as the Thecostraca barnacles (the most diverse group of crustaceans), Aeglidae freshwater crabs (the most abundant freshwater crustaceans in South America) and the Lumbricidae earthworms (the most abundant invertebrate in agricultural lands) using molecular and morphological data. Those evolutionary hypotheses led to the discovering of many new species, suggested important taxonomic rearrangements and promoted new classifications, evaluated their conservation status, and explained the evolution of key morphological features and lifestyles (e.g., parasitism). Moreover, in these studies we have tested the performance of several likelihood and Bayesian approaches for estimating molecular divergence times. We have also studied the processes and factors that may be responsible for the contemporary geographic distributions of individuals (phylogeography). In this regard, my research has contributed to assess the impact of past and present geological and climatic events on the evolutionary history of freshwater fauna in Patagonia and of marine invertebrates in the Atlantic-Mediterranean region.

Phylodynamics of Infectious Diseases

We have studied the genetic diversity, molecular evolution and dynamics of Neisseria gonorrhoeae and HIV-1 in USA, China and Thailand. In these studies we have also compared methods of pathogen identification (typing) and population dynamics. My research has revealed the relative impact of evolutionary forces, social and demographic factors, and antibiotic usage practices on the evolution of gonorrhea and suggested better control and prevention strategies. My HIV research has showed innate differences in immune response among American ethnicities, assessed the impact of vaccination and prevention campaigns on disease evolution, and pointed out a high risk of HIV resurgence in USA and Thailand despite recession of the epidemic.

Earthworm-Microbe Interactions

Decomposition is one of the key processes regulating nutrient cycling in terrestrial ecosystems and is mainly carried out by microorganisms (protists, bacteria and archaea). When present, earthworms play a critical role in decomposition by modifying the organic matter and the microorganisms that pass through the earthworms’ gut. In fact, the earthworm gut has been described as a “mutualistic digestive system” wherein host and microbes contribute to and benefit from the degradation of ingested material; however the composition of the Lumbricoidea microbiota(s), the impact gut passage has on microbial diversity and function, and the nature of this synergetic association (mutualism, commensalism, competition) remains to be determine. Similarly, how this microfauna is ontogenicaly transmitted (vertically vs horizontal) and possible coevolution (or their lack of) with their hosts remains to be determined. My future goal is to use next-generation sequencing methodologies (metagenomics and metatranscriptomics) to study the interaction between Lumbricoidea earthworms and their microbes within an evolutionary framework. 

Education

B.S. University of Santiago de Compostela, Spain, 1992 (Biological Sciences)

M.S. University of Vigo, Spain, 1993 (Biological Sciences)

Ph.D. University of Vigo, Spain, 1998 (Biological Sciences)

Publications

Scientific Journals

2015     Dominguez J., M. Aira, J. W. Breinholt, M. Stojanovic, S. W. James, M. Pérez-Losada. Underground evolution: new roots for the old tree of lumbricid earthworms. Molecular Phylogenetics and Evolution 83: 7–19.

Pérez-Losada, M., J. W. Breinholt, M. Aira, J. Domínguez. An updated multilocus phylogeny of the Lumbricidae (Annelida: Clitellata: Oligochaeta) earthworms. Journal of Phylogenetics & Evolutionary Biology 3:1.

M. Pérez-Losada, M., M. Arenas, J. C. Galán, F. Palero; F. Gonzalez-Candelas. Recombination in viruses: Mechanisms, methods of study, and evolutionary consequence. Infection, Genetics and Evolution 30: 296-307.

Marcos Pérez-Losada, Eduardo Castro-Nallar, Matthew Bendall, Robert J. Freishtat, Keith A. Crandall. Dual transcriptomic profiling of host and microbiota during health and disease in pediatric asthma. PLoS One 10(6): e0131819.

Römbke Jörg; Manuel Aira; Thierry Backeljau; Karin Breugelmans; Jorge Dominguez; Elisabeth Funke; Nadin Graf; Mehrdad Hajibabaei; Marcos Perez-Losada; Pablo G Porto; Rüdiger Schmelz; Joaquin Vierna; Anton Vizcaino; Markus Pfenninger. DNA barcoding of earthworms (Eisenia fetida/andrei) from 28 ecotoxicological test laboratories. Applied Soil Ecology (in press).

2014     Toma I., Marc O. Siegel, John Keiser, Anna Yakovleva, Alvin Kim, Lionel Davenport, Joseph Devaney, Eric P. Hoffman, Rami Alsubai, Keith A. Crandall, Eduardo Castro-Nallar, Marcos Pérez-Losada, Sarah Hilton, Lakhmir S. Chawla, Timothy A. McCaffrey, and Gary L. Simon. Single-Molecule Long Read 16S Sequencing to Characterize the Lung Microbiome from Mechanically Ventilated Patients with Suspected Pneumonia. Journal of Clinical Microbiology 52: 3913–3921.

Pérez-Losada M., Jens T. Høeg, Noa Simon-Blecher, Yair Achituv, Diana Jones, Keith A. Crandall. Molecular phylogeny, systematics and morphological evolution of the acorn barnacles (Thoracica: Sessilia: Balanomorpha). Molecular Phylogenetics and Evolution 81:147–158.

 

Pérez-Losada, M., P. Cabezas, E. Castro-Nallar, K. A. Crandall. 2013. Pathogen typing in the genomics era: MLST and the future of molecular epidemiology. Infection, Genetics and Evolution 16: 38-53.

Xavier R. , Severino R., Pérez-Losada M., Cable J., Harris D.J. 2013. First record of Sphaerospora dicentrarchi (Myxosporea, Sphaerosporidae) in Dicentrarchus punctatus. Bulletin of the European Association of Fish Pathologists 33: 21-24.

Wetzer, R., M. Pérez-Losada & N. L. Bruce. 2013. Phylogenetic relationships of the family Sphaeromatidae Latreille, 1825 (Crustacea: Peracarida: Isopoda) within Sphaeromatidea based on 18S-rDNA molecular data. Zootaxa 3599: 161–177.

Castellanos-Martínez, S., M. Pérez-Losada & C. Gestal. 2013. Molecular phylogenetic analysis of the coccidian cephalopod parasites Aggregata octopiana and Aggregata eberthi from the NE Atlantic coast using 18S rRNA sequences. European Journal of Parasitology 49: 373–380.

Faria J., E. Froufe, F. Tuya, P. Alexandrino & M. Pérez-Losada. 2013. Panmixia in the endangered slipper lobster Scyllarides latus from the Northeastern Atlantic and Western Mediterranean. Journal of Crustacean Biology 33: 557-566.

Andonova, A., M. B. Coulthart, M. Pérez-Losada, K. A. Crandall, D. Posada, R. Padmore, A. Giulivi, J. J. Oger, A. A. Peters, G. A. Dekaban. 2012. Insights into origins of Human T-cell Lymphotropic Virus Type 1 based on new strains from aboriginal people of Canada. Infection, Genetics and Evolution 12: 1822–1830.

Pérez-Losada, M., J. T. Høeg, K. A. Crandall & Y. Achituv. 2012. Molecular phylogeny and character evolution of the chthamaloid barnacles (Cirripedia: Thoracica). Molecular Phylogenetics and Evolution 65: 329-334.

Barber, B., J. Xu, M. Pérez-Losada, C. G. Jara & K. A. Crandall. 2012. Conflicting evolutionary patterns due to mitochondrial introgression and multilocus phylogeography of the Patagonian freshwater crab Aegla neuquensis. PLoS ONE 7: e37105.

Pérez-Losada, M., J. T. Høeg, Y. Achituv & K. A. Crandall 2012. Deep phylogeny and character evolution in the Thecostraca barnacles. Integrative and Comparative Biology (accepted).

Santos, S., G. Bond-Buckup, L. Buckup, M. Pérez-Losada, M. Finley, K. A. Crandall 2012. Three new species of Aegla (ANOMURA) freshwater crabs from the upper Uruguay River hydrographic basin in Brazil. Journal of Crustacean Biology (in press).

Santos, S., G. Bond-Buckup, L. Buckup, M. Pérez-Losada, M. Finley, K. A. Crandall. 2012. Three new species of Aegla (ANOMURA) freshwater crabs from the upper Uruguay River hydrographic basin in Brazil. Journal of Crustacean Biology 32: 529-540.

Barber, B. R., P. J. Unmack, M. Pérez-Losada, J. B. Johnson and K.A. Crandall 2011. Different processes lead to identical patterns: a test of codivergence and the role of sea-level and climate changes in shaping a southern temperate species assemblage. BMC Evolutionary Biology 11: 343.

Castro-Nallar, E., K. A. Crandall & M. Pérez-Losada 2012. Genetic diversity and molecular epidemiology of HIV transmission. Future Virology 7: 1-14.

Pérez-Losada, M., R. Bloch, J. W. Breinholt, M. Pfenninger and J. Dominguez 2012. Taxonomic assessment of Lumbricidae (Oligochaeta) earthworm genera using DNA barcodes. European Journal of Soil Biology 48: 41-47.

Castro-Nallar, E., M. Pérez-Losada, G. F. Burton & K. A. Crandall 2012. The evolution of HIV: Inferences using phylogenetics. Molecular Phylogenetics and Evolution 62: 777-792.

Pérez-Losada, M., D. V. Jobes, F. Sinangil, K. A. Crandall, M. Arenas, D. Posada, and P. W. Berman, 2011. Phylodynamics of HIV-1 from a Phase III AIDS Vaccine Trial in Bangkok, Thailand. PLoS ONE 6: e16902.

Tazi L, Imamichi H, Hirschfeld S, Metcalf JA, Orsega S, Pérez-Losada M, Posada D, Clifford Lane H & Crandall KA, 2011. HIV-1 infected monozygotic twins: a tale of two outcomes. BMC Evolutionary Biology 11:62.

Froufe, E., P. Cabezas, P. Alexandrino, and M. Pérez-Losada. Comparative phylogeography of three achelate lobster species from Macaronesia (North East Atlantic). Phylogeography and Population Genetics in Crustacea. Crustacean Issues 19: 157-173.

Pérez-Losada, M., J. Xu, C. Jara and K. Crandall. Comparing phylogeographic patterns across the Patagonian Andes in two freshwater crabs of the genus Aegla (Decapoda: Aeglidae). Phylogeography and Population Genetics in Crustacea. Crustacean Issues 19: 291-303.

Toon, A., M. Pérez-Losada, C. E. Schweitzer, R. M. Feldman, M. Carlson & K. A. Crandall. Gondwanan radiation of the southern hemisphere crayfish (Decapoda: Parastacidae): Evidence from fossils and molecules. Journal of Biogeography 37: 2275–2290.

Bond-Buckup, G., C. G. Jara, L. Buckup, M. Pérez-Losada, A. A. P. Bueno, K. A. Crandall & S. Santos, 2010. New species and new records of endemic freshwater crabs from the Atlantic forest in southern Brazil (Anomura, Aeglidae). Journal of Crustacean Biology 30: 495-502.

Nuñez, J. J., M. T. González & M. Pérez-Losada, 2010. Testing species boundaries between Sebastes capensis and S. oculatus (Teleostei: Scorpaenidae) through mitochondrial DNA sequences. Revista de Biología Marina y Oceanografía 45: S1 565-573.

Santos S, Bond-Buckup G, Pérez-Losada M., Jara CG, Crandall KA & Buckup L, 2010. New records and description of a new species of Aeglidae (Crustacea: Anomura) from river basins in Southern Brazil. Nauplius 18: 79-86.

Guerra, A., A. Roura, A. F. González, S. Pascual, Y. Cherel & M. Pérez-Losada, 2010. Morphological and genetic evidence that Octopus vulgaris Cuvier, 1797 inhabits Amsterdam and Saint Paul Islands (Southern Indian Ocean). ICES Journal of Marine Science 67: 1401-1407.

Bond-Buckup, G, C. G. Jara, L. Buckup, A. A. P. Bueno, M. Pérez-Losada & K. A. Crandall, 2010. Description of a new species of Aeglidae, and new records of related species from river basins in Argentina (Crustacea, Anomura). Zootaxa 2343: 18-30.

Tazi, T., M. Pérez-Losada, W. Gu, L. Xue, K. A. Crandall & R. P. Viscidi, 2010. Population Dynamics of Neisseria gonorrhoeae in Shanghai, China: A Comparative Study. BMC Infectious Diseases 10: 13.

Pérez-Losada, M., David V. Jobes, Faruk Sinangil, Keith A. Crandall, David Posada, and Phillip W. Berman, 2010. Phylodynamics of gp120 sequences from a Phase 3 HIV-1 vaccine trial in North America. Molecular Biology and Evolution 27: 417-425.

Jens T. Høeg, Marcos Perez-Losada, Henrik Glenner, Gregory A Kolbasov and Keith A. Crandall, 2009. Evolution of morphology, ontogeny and life cycles within the Crustacea Thecostraca. Arthropod Systematics and Phylogeny 67: 199-217.

Santos, S., G. Bond-Buckup, M. Pérez-Losada, M. Ladvocat Bartholomei-Santos & L. Buckup, 2009. Aegla manuinflata, a new species of freshwater anomuran (Decapoda: Anomura: Aeglidae) from the Ibicuí River basin, Rio Grande do Sul, Brazil, determined by morphological and molecular characters. Zootaxa 2088: 31-40.

Pérez-Losada, M., Jens T. Høeg and Keith A. Crandall, 2009. Remarkable convergent evolution in specialized parasitic Thecostraca (Crustacea). BMC Biology 7: 15.

Pérez-Losada, M., D. Posada, D. V. Jobes, F. Sinangil, P. W. Berman & K. A. Crandall, 2009. Ethnic differences in the adaptation rate of HIV gp120 from a vaccine trail. Retrovirology 6: 67.

Pérez-Losada, M., M. Ricoy, J. Marshall & J. Domínguez, 2009. Phylogenetic assessment of the earthworm Aporrectodea caliginosa species complex (Oligochaeta, Lumbricidae) based on mitochondrial and nuclear DNA sequences. Molecular Phylogenetics and Evolution 52: 293-302.

Xu, J., M. Pérez-Losada, C. G. Jara & K. A. Crandall, 2009. Pleistocene glaciation leaves deep signature on the freshwater crab Aegla alacalufi in Chilean Patagonia. Molecular Ecology 18: 904-918.

Pérez-Losada, M., G. Bond-Buckup, C. G. Jara & K. A. Crandall, 2009. Conservation assessment of southern South American freshwater ecoregions on the basis of the distribution and genetic diversity of crabs from the genus Aegla. Conservation Biology 23: 692-702.

Høeg, J. T., Y. Achituv, B. K.K. Chan, K. Chan, P. Gram Jensen & M. Pérez-Losada, 2009. Cypris Morphology in the barnacles Ibla and Paralepas (Crustacea: Cirripedia Thoracica) - Implications for Cirripede Evolution. Journal of Morphology 270: 241-255.

Weaver, K. F., M. Pérez-Losada, R. P. Guralnick, A. Nelson, S. Blatt & K. A. Crandall, 2008. Assessing the conservation status of the land snail Oreohelix peripherica wasatchensis (Family Oreohelicidae). Conservation Genetics 9: 907-916.

Carvajal-Rodríguez, A., D. Posada, M. Pérez-Losada, E. Keller, E. J. Abrams, K. A. Crandall and R. P. Viscidi. 2008. Disease progression and evolution of the HIV-1 env gene in 24 infected infants. Infection Genetics and Evolution 8:110-120.

Bond-Buckup, G., C. G. Jara, M. Pérez-Losada, L. Buckup & K. A. Crandall, 2008. An assessment of global diversity of the freshwater ‘crab’ Aeglidae (Decapoda: Anomura). Hydrobiologia 595: 267–273.

Høeg, J., H. Glenner, M.J. Grygier, Y. Fujita, M. Pérez-Losada and K.A. Crandall, 2008. Metamorphosis in crustacean Y-larvae. A parasitic solution to an old zoological riddle. Journal of Morphology 269: 1471.

Domínguez, J., M. Ricoy, and M. Pérez-Losada. 2008. Eisenia fetida (Savigny, 1826) y Eisenia andrei Bouché, 1972 son dos especies diferentes de lombrices de tierra. Acta Zoologica Mexicana 2: 321-331.

Pérez-Losada, M., M. Harp, J. T. Høeg, Y. Achituv, D. Jones, H. Watanabe & K. A. Crandall, 2008. The tempo and mode of barnacle evolution. Molecular Phylogenetics and Evolution 46: 328–346.

Porter, M. L., K. Dittmar de la Cruz & M. Pérez-Losada, 2007. How long does evolution of the troglomorphic form take? Estimating divergence times in Astyanax mexicanus. Acta Carsologica 36: 173-182.

Pérez-Losada, M., K. A. Crandall, M. C. Bash, M. Dan, J. Zenilman & R. P. Viscidi, 2007. Molecular Epidemiology of Quinolone-Resistant Neisseria gonorrhoeae in Israel. BMC Evolutionary Biology 7:84-94.

Pérez-Losada, M., K. A. Crandall, J. Zenilman & R. P Viscidi, 2007. Temporal trends in gonococcal population genetics in a high prevalence urban community. Infection, Genetics and Evolution 7:271-278.

Pérez-Losada, M., M. J. Nolte, K. A. Crandall, & P. W. Shaw, 2007. Testing hypotheses of population structuring in the NE Atlantic Ocean and Mediterranean Sea using the common cuttlefish Sepia officinalis. Molecular Ecology 16:2667-2679.

Pérez-Losada, M., M. L. Porter, L. Tazi, & K. A. Crandall, 2007. New methods for inferring population dynamics from microbial sequences. Infection, Genetics and Evolution 7:24-43.

Crandall, K. A., M. Pérez-Losada, R. G. Christensen, D. A. McClellan & R. P. Viscidi, 2006. Phylogenomics and molecular evolution of Polyomaviruses. Advances in Experimental Medicine and Biology 577:46-59. Review.

Jobes, D. V., M. Daoust, V. T. Nguyen, A. Padua, F. Sinangil, M. Pérez-Losada, K. A. Crandall, T. Oliphant, D. Posada, A. Rambaut, J. Fuchs, & P. W. Berman. 2006. Longitudinal population analysis of dual infection with recombination in two strains of HIV-1 subtype B in an individual from a Phase 3 HIV vaccine efficacy trial. AIDS 22:968-978.

Pérez-Losada, M., R. G. Christensen, D. A. McClellan, Byron Adams, R. P. Viscidi, and K. A. Crandall, 2006. Phylogenetic codivergence of polyomaviruses and their hosts. Journal of Virology 80: 5663-5669.

Daniels, S. R., N. Cumberlidge, M. Pérez-Losada & K. A. Crandall, 2006. Evolution of Afrotropical freshwater crab lineages obscured by morphological convergence. Molecular Phylogenetics and Evolution 40:227-235.

Pérez-Losada, M., E. B. Browne, A. Madsen, T. Wirth, R. P. Viscidi & K. A. Crandall, 2006. Population genetics of microbial pathogens estimated from Multilocus Sequence Typing (MLST) data. Infection, Genetics and Evolution 6:97-112.

Martínez, P., M. Pérez-Losada, A. Guerra & A. Sanjuan, 2005. First genetic validation and diagnosis of the short-finned squid species of the genus Illex (Cephalopoda: Ommastrephidae). Marine Biology 148:97-108.

Porter, M. L., M. Pérez-Losada & K .A. Crandall, 2005. Model based multi-locus estimation of Decapod phylogeny and divergence times. Molecular Phylogenetics and Evolution 37:355-369.

Pérez-Losada, M., Raphael P. Viscidi, & Keith A. Crandall, 2005. Population Genetics of Neisseria gonorrhoeae in a High Prevalence Community Using a Hyper-Variable Outer Membrane porB and Thirteen Slow-Evolving Housekeeping Genes. Molecular Biology and Evolution 22: 1887-1902.

Shull, H. C., M. Pérez-Losada, D. Blair, K. Sewell, E. A. Sinclair, S. Lawler, M. Ponniah & K. A. Crandall, 2005. Phylogeny and biogeography of the freshwater crayfish Euastacus (Decapoda: Parastacidae) based on nuclear and mitochondrial DNA. Molecular Phylogenetics and Evolution 37: 249-263.

Pérez-Losada, M., J. Eiroa, S. Mato & J. Domínguez, 2005. Phylogenetic species delimitation of the earthworms Eisenia fetida (Savigny, 1826) and Eisenia andrei Bouché, 1972 (Oligochaeta, Lumbricidae) based on mitochondrial and nuclear DNA sequences. Pedobiologia 49:317-324.

Pérez-Losada M., G. Bond-Buckup, C. Jara & K. A. Crandall, 2004. Molecular systematics and biogeography of the southern South American freshwater “crabs” Aegla (Decapoda: Anomura: Aeglidae) using multiple heuristic tree search approaches. Systematic Biology 53: 767-780.

Pérez-Losada M., J. T. Høeg & K. A. Crandall, 2004. Unraveling the evolutionary radiation of the thoracican barnacles using molecular and morphological evidence: a comparison of several divergence time estimation approaches. Systematic Biology 53: 244-264.

Pérez-Losada M. & K. A. Crandall, 2003. Can taxonomic richness be used as a surrogate for phylogenetic distinctness indices for ranking areas for conservation? Animal Biodiversity and Conservation 26.1: 77-84.

Jara C. G., M. Pérez-Losada & K. A. Crandall, 2003. Aegla occidentalis (Crustacea: Decapoda: Aeglidae), a new species of freshwater anomuran crab from the Nahuelbuta Coastal Range, Chile. Proceeding of the Biological Society of Washington 116: 954-963.

Pérez-Losada M., A. Guerra & A. Sanjuan, 2002. Allozyme divergence supporting the taxonomic separation of Octopus mimus and O. maya from Octopus vulgaris (Cephalopoda: Octopoda). Bulletin of Marine Science 71: 653-664.

Pérez-Losada M., A. Guerra, G. R. Carvalho, A. Sanjuan & P. W. Shaw, 2002. Extensive population subdivision of the cuttlefish Sepia officinalis (Mollusca: Cephalopoda) around the Iberian Peninsula indicated by microsatellite DNA variation. Heredity 89: 417-424.

Pérez-Losada M., C. Jara, G. Bond-Buckup & K. A. Crandall, 2002. Phylogenetic relationships among the species of Aegla (ANOMURA: AEGLIDAE) freshwater crabs from Chile. Journal of Crustacean Biology 22: 304-313.

Pérez-Losada M., J. T. Høeg, G. A. Kolbasov & K. A. Crandall, 2002. Reanalysis of the relationships among the Cirripedia and the Ascothoracida and the phylogenetic position of the Facetotecta (Maxillopoda: Thecostraca) using 18S rDNA sequences. Journal of Crustacean Biology 22: 661-669.

Pérez-Losada M., C. Jara, G. Bond-Buckup, M. Porter & K. A. Crandall, 2002. Phylogenetic position of the freshwater anomuran Aeglidae. Journal of Crustacean Biology 22: 670-676.

Pérez-Losada M., C. Jara, G. Bond-Buckup & K. A. Crandall, 2002. Conservation phylogenetics of Chilean freshwater crabs Aegla (ANOMURA, AEGLIDAE): assigning priorities for aquatic habitat protection. Biological Conservation 105: 345-353.

Guerra A., M. Pérez-Losada, F. Rocha & A. Sanjuan, 2001. Species differentiation of Sepia officinalis and Sepia hierredda (Cephalopoda: Sepiidae) based on morphological and allozyme analyses. Journal Marine Biology Association U.K. 81: 271-281.

Shaw P. W. & M. Pérez-Losada, 1999. Polymorphic microsatellites in the common cuttlefish Sepia officinalis. Molecular Ecology 9: 237-238.

Pérez-Losada M., A. Guerra & A. Sanjuan, 1999. Allozyme differentiation in the cuttlefish Sepia officinalis (Mollusca: Cephalopoda) from the NE Atlantic and Mediterranean. Heredity 83: 280-289.

Sanjuan A., M. Pérez-Losada & E. Rolan, 1997. Allozyme evidence for cryptic speciation in sympatric populations of the marine pollution indicator Nassarius spp. (MOLLUSCA: GASTROPODA). Journal Marine Biology Association U. K. 77: 773-784.

Sanjuan A., M. Pérez-Losada & A. Guerra, 1996. Genetic differentiation in three Sepia species (MOLLUSCA: CEPHALOPODA) from Galician waters (northwest Iberian Peninsula). Marine Biology 126: 253-259.

Pérez-Losada M., A. Guerra & A. Sanjuan, 1996. Allozyme electrophoretic technique and phylogenetic relationships in three species of Sepia (CEPHALOPODA: SEPIIDAE). Comparative Biochemical Physiology 114B: 11-18.

Book Chapters

Pérez-Losada, M., M. L. Porter, R. P. Viscidi, and K. A. Crandall, 2011. Multilocus sequence typing of pathogens. In: Genetics and evolution of infectious diseases, pp. 503-521 (M. Tibayrenc, Ed). Elsevier Inc.

Pérez-Losada, M. 2011. Mandibulados. In: El árbol de la vida: sistemática y evolución de los seres vivos, chapter 27 (Rafael Zardoya and P. Vargas-Gómez, Eds.). Museo de Ciencias Naturales, CSIC, Madrid, Spain (in press).

Breinholt, J, M. Pérez-Losada & K. A. Crandall, 2009. The timing of the diversification of the freshwater crayfishes. In: Decapoda crustacean phylogenetics, pp. 335-347 (J. W. Martin, K. A. Crandall and  D. L. Felder, Eds.). CRC Press, Taylor & Francis Group.

Pérez-Losada, M., J. T. Høeg and K. A. Crandall, 2009. Stalked and acorn barnacles (Thoracica). In: Time tree of Life, pp. 298-301 (S. Blair Hedges and Sudhir Kumar, Eds.). Oxford University Press, Oxford.

Crandall, K. A., M. L. Porter, M. Pérez-Losada, 2009. Crabs, shrimps and lobsters (Decapoda). In: Time tree of Life, pp. 293-297 (S. Blair Hedges and Sudhir Kumar, Eds.). Oxford University Press, Oxford.

Pérez-Losada, M., M. L. Porter & K. A. Crandall, 2008. Methods for analyzing viral evolution. In: Plant Virus Evolution, pp. 165-204 (M. J. Roossinck, Ed.). Springer-Verlag Berlin Heidelberg.

Crandall, K. A. & M. Pérez-Losada, 2008. Epidemiological and evolutionary dynamics of pathogens. In: Evolutionary biology of bacterial and fungal pathogens, pp. 21-30 (Baquero et al., Eds.). American Society of Microbioly Press, Washington, D. C.

Sinclair E. A., M. Pérez-Losada & K. A. Crandall, 2005. Molecular phylogenetics for conservation biology. In Phylogeny and conservation, pp. 19-56 (Purvis, A., T. Brooks, and J. Gittleman, Eds.). Cambridge University Press, Cambridge, UK.

Distinctions

Thesis Award, University of Vigo, Spain